Antibiotic Resistance Patterns of Pseudomonas aeruginosa Bacterial Species Isolated from Various Clinical Samples: Antibiotic Resistance of Pseudomonas aeruginosa

Qandeel Abbass Soomro; Abdul Nabi; Sarfraz Ali Tunio; Abdul Sattar Qureshi; Nazir Ahmed Brohi; Faryal Ahmed Khushik; Abdul Sami Dahri; Asim Patrick

doi:10.54393/pjhs.v5i09.1910

Authors

Qandeel Abbass Soomro Department of Microbiology and Pathology, Indus Medical College, Tando Muhammad Khan, Pakistan
Abdul Nabi Institute of Microbiology, University of Sindh, Jamshoro, Pakistan
Sarfraz Ali Tunio Institute of Microbiology, University of Sindh, Jamshoro, Pakistan
Abdul Sattar Qureshi Institute of Biotechnology and Genetic Engineering, University of Sindh, Jamshoro, Pakistan
Nazir Ahmed Brohi Institute of Microbiology, University of Sindh, Jamshoro, Pakistan
Faryal Ahmed Khushik Institute of Microbiology, University of Sindh, Jamshoro, Pakistan
Abdul Sami Dahri Department of Microbiology, Government College University Hyderabad, Hyderabad, Pakistan
Asim Patrick Department of Microbiology, Government College University Hyderabad, Hyderabad, Pakistan

DOI:

https://doi.org/10.54393/pjhs.v5i09.1910

Keywords:

Pseudomonas aeruginosa, Antibiotic Resistance, Multidrug-Resistance

Abstract

Pseudomonas aeruginosa infections have become a real burden in healthcare settings, contributing substantially to nosocomial infections. The emergence of several drug-resistant strains was the major issue causing massive mortality. Multiple-drug resistant Pseudomonas aeruginosa isolates containing beta-lactamase were becoming more prevalent. Objective: To investigate and characterize the antibiotic resistance patterns of Pseudomonas aeruginosa isolated from different clinical samples. Methods: A total of 618 different clinical samples including blood, pus and urine samples were collected from the patients visiting Indus Medical College Hospital Tando Muhammad Khan, Sindh, Pakistan. All the clinical samples were processed for the isolation of bacterial species using specific culture media. The identification of Pseudomonas aeruginosa strains was achieved based on Gram’s staining and certain biochemical tests including Cetrimide test. Moreover, antibiotic susceptibility testing was determined by Kirby-Bauer disc diffusion method. Results: A total of 452 bacterial strains were isolated and pure cultured from different clinical samples. Among these, 60.62% were Gram-negative and 25% of the total isolates were found to be Pseudomonas aeruginosa strains. Antibiotic sensitivity testing results revealed the highest resistance ratio of Pseudomonas aeruginosa strains against Ticarcillin (46.02%), while Colistin showed the lowest resistance (3.54%). Overall, 14.15% of the isolates of Pseudomonas aeruginosa strains were found to be multi-drug-resistant bacteria. Conclusion: The results of the present study reveal an increased ratio of antibiotic resistance particularly of Ticarcillin, piperacillin and imipenem in Pseudomonas aeruginosa isolated from various clinical samples.

References

Jenny M and Kingsbury J. Properties and prevention: A review of Pseudomonas aeruginosa. Journal of Biological and Medical Research. 2018 Sep; 2(3): 1-8.

Nisarta A and Rajat R. Detection and Antibiotic Susceptibility Pattern of Pseudomonas Aeruginosa Isolates from Various Clinical Samples in Tertiary Care Hospital, Bhavnagar, Gujarat. 2019.

Said SA, Khalid HM, Mero WM. Prevalence of Pseudomonas aeruginosa Isolates and their Antibiotic Susceptibility among Patients and Healthcare Workers in Three Hospitals of Duhok City/Iraq. Journal of Contemporary Medical Sciences. 2023 Sep; 9(5). doi: 10.22317/jcms.v9i5.1428. DOI: https://doi.org/10.22317/jcms.v9i5.1428

Bonyadi P, Saleh NT, Dehghani M, Yamini M, Amini K. Prevalence of antibiotic resistance of Pseudomonas aeruginosa in cystic fibrosis infection: A systematic review and meta-analysis. Microbial Pathogenesis. 2022 Apr; 165: 105461. doi: 10.1016/j.micpath.2022.105461. DOI: https://doi.org/10.1016/j.micpath.2022.105461

Karami P, Khaledi A, Mashoof RY, Yaghoobi MH, Karami M, Dastan D et al. The correlation between biofilm formation capability and antibiotic resistance pattern in Pseudomonas aeruginosa. Gene Reports. 2020 Mar; 18: 100561. doi: 10.1016/j.genrep.2019.100561. DOI: https://doi.org/10.1016/j.genrep.2019.100561

Abbas A, Khan L, Muzammil HS, Aftab MM. Sensitivity and Resistivity of Various Antibiotics Against of Pseudomonas aeruginosa in Clinical Isolates: Antibiotics Against Clinical Isolates of Pseudomonas aeruginosa. Futuristic Biotechnology. 2021 Jun: 25-8. doi: 10.54393/fbt.v1i01.7. DOI: https://doi.org/10.54393/fbt.v1i01.7

Moreau-Marquis S, Redelman CV, Stanton BA, Anderson GG. Co-culture models of Pseudomonas aeruginosa biofilms grown on live human airway cells. Journal of Visualized Experiments. 2010 Oct; 6(44): e2186. doi: 10.3791/2186. DOI: https://doi.org/10.3791/2186-v

Crabbé A, Liu Y, Matthijs N, Rigole P, De La Fuente-Nùñez C, Davis R et al. Antimicrobial efficacy against Pseudomonas aeruginosa biofilm formation in a three-dimensional lung epithelial model and the influence of fetal bovine serum. Scientific Reports. 2017 Mar; 7(1): 43321. doi: 10.1038/srep43321. DOI: https://doi.org/10.1038/srep43321

Jyothi P, Shahapur PR, Metri BC. Antibiotic Resistance among Pseudomonas aeruginosa Isolated in Tertiary Care Hospital, Vijayapur, India. 2020; 4(9): 2319-7706. doi: 10.20546/ijcmas.2020.904.088. DOI: https://doi.org/10.20546/ijcmas.2020.904.088

Paudel P, Shrestha S, Poudel S, Tiwari BR. Multi-drug resistant bacteria isolated from pus and phenotypic detection of metallo β-lactamase activity of Pseudomonas aeruginosa. Nepal Journal of Health Sciences. 2021 Jul; 1(1): 8-15. doi: 10.3126/njhs.v1i1.38604. DOI: https://doi.org/10.3126/njhs.v1i1.38604

Kanthakumar A and Jayavarthinni M. Antibiotic resistance pattern of pseudomonas aeruginosa isolated from various clinical samples in a tertiary care hospital, puducherry. Asian Journal of Pharmaceutical and Clinical Research. 2022 Feb; 15(2): 106-8. doi: 10.22159/ajpcr.2022.v15i2.43839. DOI: https://doi.org/10.22159/ajpcr.2022.v15i2.43839

Panwar R, Kumari P, Basant. Antimicrobial drug resistance profiling of pseudomonas aeruginosa isolated from nosocomial infections. International Journal of Advanced Biochemistry Research.2024 Jan; 8(1): 569-72. doi:10.33545/26174693.2024.v8.i1h.440. DOI: https://doi.org/10.33545/26174693.2024.v8.i1h.440

Debnath J, Saha A, Das PK, Das NS, Saha S. Antibiotic Susceptibility Pattern in Clinical Isolates of Pseudomonas aeruginosa from a Tertiary Care Hospital of Tripura. International Journal of Current Microbiology and Applied Sciences. Int. 2019; 8(3): 291-8. doi: 10.20546/ijcmas.2019.803.036. DOI: https://doi.org/10.20546/ijcmas.2019.803.036

PA W. Clinical and Laboratory Standards Institute: Performance standards for antimicrobial susceptibility testing: 20th informational supplement. CLSI document M100-S20. 2010.

Qayoom S, Rashid A, Kohli A, Masoodi T, Amin M. Prevalence and antibiotic sensitivity pattern of Pseudomonas aeruginosa isolates from respiratory samples, pus samples and body fluids in a tertiary care hospital, Kashmir. Indian Journal of Microbiology Research. 2019; 6(4): 345-9. doi: 10.18231/j.ijmr.2019.073. DOI: https://doi.org/10.18231/j.ijmr.2019.073

Sannathimmappa MB, Nambiar V, Aravindakshan R. A 5-Year Retrospective Study of Antibiotic Resistance Pattern of Pseudomonas aeruginosa Isolated from Various Clinical Samples at a Tertiary Care Hospital. Medical Journal of Dr. DY Patil University. 2023 Sep; 16(5): 687-92. doi: 10.4103/mjdrdypu.mjdrdypu_312_21. DOI: https://doi.org/10.4103/mjdrdypu.mjdrdypu_312_21

Mahaseth SN, Chaurasia L, Jha B, Sanjana RK. Prevalence and antimicrobial susceptibility pattern of Pseudomonas aeruginosa isolated from various clinical samples in a tertiary Care Hospital. Janaki Medical College Journal of Medical Science. 2020 Dec; 8(2): 11-7. doi: 10.3126/jmcjms.v8i2.33972. DOI: https://doi.org/10.3126/jmcjms.v8i2.33972

Odoi H, Boamah VE, Duah Boakye Y, Dodoo CC, Agyare C. Sensitivity patterns, plasmid profiles and clonal relatedness of multi-drug resistant Pseudomonas aeruginosa isolated from the Ashanti region, Ghana. Environmental Health Insights. 2022 Feb; 16: 11786302221078117. doi: 10.1177/11786302221078117. DOI: https://doi.org/10.1177/11786302221078117

Ahmad S, Alotaibi MA, Alamri MS. Antibiotic sensitivity pattern of clinical isolates of Pseudomonas aeruginosa at a tertiary care hospital in Saudi Arabia. Dhaka University Journal of Pharmaceutical Sciences. 2020 Jun; 19(1): 77-82. doi: 10.3329/dujps.v19i1.47821. DOI: https://doi.org/10.3329/dujps.v19i1.47821

Farooq L, Memon Z, Ismail MO, Sadiq S. Frequency and antibiogram of multi-drug resistant pseudomonas aeruginosa in a Tertiary Care Hospital of Pakistan. Pakistan Journal of Medical Sciences. 2019 Nov; 35(6): 1622. doi: 10.12669/pjms.35.6.930. DOI: https://doi.org/10.12669/pjms.35.6.930

Zahoor S, Mahboob HM, Bukhari H, Ali A, Jehangir HM, Siddiqui A. Frequency and Antimicrobial Susceptibility Pattern of Pseudomonas aerugonisa Clinical Isolates at a Large Teaching Hospital In Lahore, Pakistan. Pakistan Postgraduate Medical Journal. 2020; 31(04): 168-71. doi: 10.51642/ppmj.v31i04.366. DOI: https://doi.org/10.51642/ppmj.v31i04.366

Walaa MS, Samah G, Hatem M, Nikhat M. In vitro antibiotic resistance patterns of Pseudomonas spp. isolated from clinical samples of a hospital in Madinah, Saudi Arabia. African Journal of Microbiology Research. 2018 Jan 7; 12(1): 19-26. doi: 10.5897/AJMR2017.8743. DOI: https://doi.org/10.5897/AJMR2017.8743