Variations in Gonadal Steroids in Workers Occupationally Exposed to Toxicants at Automobile Workshops and Petrol Filling Stations: Variations in Gonadal Steroids in Workers

Bilal Javed; Muhammad  Amir Iqbal; Shaaf  Ahmad; Husna  Ahmad; Nabila  Roohi

doi:10.54393/pjhs.v3i05.194

Authors

Bilal Javed Department of Zoology, University of the Punjab, Lahore, Pakistan
Muhammad Amir Iqbal Department of Zoology, University of the Punjab, Lahore, Pakistan
Shaaf Ahmad King Edward Medical University/Mayo Hospital, Lahore, Pakistan
Husna Ahmad Department of Zoology, University of the Punjab, Lahore, Pakistan
Nabila Roohi Department of Zoology, University of the Punjab, Lahore, Pakistan

DOI:

https://doi.org/10.54393/pjhs.v3i05.194

Keywords:

LH, FSH, Testosterone, Estradiol, Steroids

Abstract

The most at risk for occupational toxicity brought on by exposure to heavy metals and PAHs among various vocations are gas station attendants and auto workers. The gonadal and its regulating hormonal pattern were identified in the current investigation in gas station attendants and car employees. Objective: This study's goal is to ascertain the impact of various occupational toxicants on the ovarian health of gasoline station attendants (PPA) and car technicians (AMM). Gonadal steroids and the hormones that control them were examined for this reason and their relationship to gonadal function was established. Methods: For this, blood samples from 19 gas station attendants and 29 auto mechanics were obtained from various gas stations and car shops, respectively. The University of the Punjab in Lahore provided the blood samples for the 24 controls. Using commercially available ELISA kits, the levels of serum estradiol, follicle stimulating hormone (FSH), luteinizing hormone (LH), and testosterone were examined. The significance of changes was evaluated using the one-way ANOVA test. Results: When compared to the control group, there was a little decrease in the levels of estradiol, follicle-stimulating hormone, luteinizing hormone, and testosterone among fuel station attendants and car employees. Conclusions: Pertinently, reduced reproductive and their regulatory hormonal levels predispose future risk of manifesting reproductive health issues.

References

Ji G, Gu A, Zhou Y, Shi X, Xia Y, Long Y, et al. Interactions between exposure to environmental polycyclic aromatic hydrocarbons and DNA repair gene polymorphisms on bulky DNA adducts in human sperm. PLoS One. 2010 Oct; 5(10): e13145. doi: 10.1371/journal.pone.0013145.

Pachathundikandi SK and Varghese ET. Blood zinc protoporphyrin, serum total protein, and total cholesterol levels in automobile workshop workers in relation to lead toxicity: Our experience. Indian Journal of Clinical Biochemistry. 2006 Sep; 21(2):114-7. doi: 10.1007/BF02912924.

Xia Y, Han Y, Zhu P, Wang S, Gu A, Wang L, et al. Relation between urinary metabolites of polycyclic aromatic hydrocarbons and human semen quality. Environmental Science & Technology. 2009 Jun; 43(12):4567-73. doi: 10.1021/es9000642.

Anetor JI, Anetor GO, Iyanda AA, Adeniyi F. Environmental chemicals and human neurotoxicity: magnitude, prognosis and markers. African Journal of Biomedical Research. 2008;11(1).

Wingfield JC, Lynn S, Soma KK. Avoiding the 'costs' of testosterone: ecological bases of hormone-behavior interactions. Brain, Behavior and Evolution. 2001 May; 57(5):239-51. doi: 10.1159/000047243.

Anawalt BD, Bebb RA, Matsumoto AM, Groome NP, Illingworth PJ, McNeilly AS, et al. Serum inhibin B levels reflect Sertoli cell function in normal men and men with testicular dysfunction. Journal of clinical endocrinology and metabolism. 1996 Sep; 81(9):3341-5. doi: 10.1210/jcem.81.9.8784094.

Keating NL, O'Malley AJ, Freedland SJ, Smith MR. Diabetes and cardiovascular disease during androgen deprivation therapy: observational study of veterans with prostate cancer. Journal of the National Cancer Institute. 2010 Jan; 102(1):39-46. doi: 10.1093/jnci/djp404.

Dyer SJ. International estimates on infertility prevalence and treatment seeking: potential need and demand for medical care. Human Reproduction. 2009 Sep; 24(9):2379-80; author reply 2380-3. doi: 10.1093/humrep/dep219.

Anetor JI and Adeniyi FA. Antioxidant status in occupational lead Toxicity in Nigeria. Biokemistri. 2001; 11:1-7.

Emokpae MA, Uadia PO, Mohammed AZ, Omale-Itodo A. Hormonal abnormalities in azoospermic men in Kano, Northern Nigeria. Indian Journal of Medical Research. 2006 Sep; 124(3):299-304.

Järup L. Hazards of heavy metal contamination. British medical bulletin. 2003; 68:167-82. doi: 10.1093/bmb/ldg032.

Okoli SU, Charles-Davies MA, Onifade AA, Adekola S. Hypogonadism in males exposed to mixed chemicals in a mechanic village in Bodija, Ibadan. Journal of Scientific Research and Reports. 2015; 8(7):1-9.

Araujo AS, Ribeiro MF, Enzveiler A, Schenkel P, Fernandes TR, Partata WA, et al. Myocardial antioxidant enzyme activities and concentration and glutathione metabolism in experimental hyperthyroidism. Molecular and Cellular Endocrinology. 2006 Apr; 249(1-2):133-9. doi: 10.1016/j.mce.2006.02.005.

Martinez CS, Escobar AG, Torres JG, Brum DS, Santos FW, Alonso MJ, et al. Chronic exposure to low doses of mercury impairs sperm quality and induces oxidative stress in rats. Journal of Toxicology and Environmental Health - Part A. 2014; 77(1-3):143-54. doi: 10.1080/15287394.2014.867202.

O'Donnell L, Robertson KM, Jones ME, Simpson ER. Estrogen and spermatogenesis. Endocrine reviews. 2001 Jun; 22(3):289-318. doi: 10.1210/edrv.22.3.0431.

Mowa CN and Iwanaga T. Expression of estrogen receptor-alpha and -beta mRNAs in the male reproductive system of the rat as revealed by in situ hybridization. Journal of molecular endocrinology. 2001 Jun; 26(3):165-74. doi: 10.1677/jme.0.0260165.

Saunders PT, Sharpe RM, Williams K, Macpherson S, Urquart H, Irvine DS, et al. Differential expression of oestrogen receptor alpha and beta proteins in the testes and male reproductive system of human and non-human primates. Molecular human reproduction. 2001 Mar;7(3):227-36. doi: 10.1093/molehr/7.3.227.

Carreau S, Bourguiba S, Lambard S, Galeraud-Denis I, Genissel C, Levallet J. Reproductive system: aromatase and estrogens. Molecular and Cellular Endocrinology. 2002 Jul; 193(1-2):137-43. doi: 10.1016/s0303-7207(02)00107-7.

Scobie GA, Macpherson S, Millar MR, Groome NP, Romana PG, Saunders PT. Human oestrogen receptors: differential expression of ER alpha and beta and the identification of ER beta variants. Steroids. 2002 Nov; 67(12):985-92. doi: 10.1016/s0039-128x (02)00047-8.

Themmen APN and Huhtaniemi IT. Mutations of gonadotropins and gonadotropin receptors: elucidating the physiology and pathophysiology of pituitary-gonadal function. Endocrine reviews. 2000 Oct; 21(5):551-83. doi: 10.1210/edrv.21.5.0409.

Dias JA, Cohen BD, Lindau-Shepard B, Nechamen CA, Peterson AJ, Schmidt A. Molecular, structural, and cellular biology of follitropin and follitropin receptor. Vitamins and Hormones. 2002; 64:249-322. doi: 10.1016/s0083-6729(02)64008-7.

Menkveld R. Clinical significance of the low normal sperm morphology value as proposed in the fifth edition of the WHO Laboratory Manual for the Examination and Processing of Human Semen. Asian journal of andrology. 2010 Jan;12(1):47.

Stephen EH and Chandra A. Declining estimates of infertility in the United States: 1982-2002. Fertility and Sterility. 2006 Sep;86(3):516-23. doi: 10.1016/j.fertnstert.2006.02.129.

Jarow JP, Espeland MA, Lipshultz LI. Evaluation of the azoospermic patient. Urology journal. 1989 Jul;142(1):62-5. doi: 10.1016/s0022-5347(17)38662-7.

Gudeloglu A and Parekattil SJ. Update in the evaluation of the azoospermic male. Clinics (Sao Paulo). 2013;(68):27-34. doi: 10.6061/clinics/2013(sup01)04.

Nedresky D and Singh G. Physiology, luteinizing hormone. In Stat Pearls [Internet] 2021 Sep. Stat Pearls Publishing.

Katsumi W. Brief Review of Luteinizing Hormone (LH, Lutropin) and Shibayagi’s Rat LH ELISA KIT. Gunma University Technical Consultant, Shibayagi Co., Ltd. 2006:1-0.

Bouwer J. The association between physical activity, blood pressure and renin in black African teachers: The SABPA Study (Doctoral dissertation, North-West University).

Johnson AR 3rd and Jarow JP. Is routine endocrine testing of impotent men necessary? Urology journal. 1992 Jun; 147(6):1542-3; discussion 1543-4. doi: 10.1016/s0022-5347(17)37620-6.

Aversa A, Isidori AM, Greco EA, Giannetta E, Gianfrilli D, Spera E, et al. Hormonal supplementation and erectile dysfunction. European Urology. 2004 May; 45(5):535-8. doi: 10.1016/j.eururo.2004.01.005.

Shabsigh R, Kaufman JM, Steidle C, Padma-Nathan H. Randomized study of testosterone gel as adjunctive therapy to sildenafil in hypogonadal men with erectile dysfunction who do not respond to sildenafil alone. Urology journal. 2008 May;179(5):97-102. doi: 10.1016/j.juro.2008.03.145.

Matsumoto AM. Andropause: clinical implications of the decline in serum testosterone levels with aging in men. Journals of Gerontology - Series A Biological Sciences and Medical Sciences. 2002 Feb; 57(2):M76-99. doi: 10.1093/gerona/57.2.m76.

Araujo AB, O'Donnell AB, Brambilla DJ, Simpson WB, Longcope C, Matsumoto AM, et al. Prevalence and incidence of androgen deficiency in middle-aged and older men: estimates from the Massachusetts Male Aging Study. Journal of clinical endocrinology and metabolism. 2004 Dec; 89(12):5920-6. doi: 10.1210/jc.2003-031719.

Carreau S, Genissel C, Bilinska B, Levallet J. Sources of oestrogen in the testis and reproductive tract of the male. Journal of andrology. 1999 Aug; 22(4):211-23. doi: 10.1046/j.1365-2605.1999.00172. x.

Hess RA. Oestrogen in fluid transport in efferent ducts of the male reproductive tract. Reviews of reproduction. 2000 May; 5(2):84-92. doi: 10.1530/ror.0.0050084.

Carreau S. Germ cells: a new source of estrogens in the male gonad. Molecular and Cellular Endocrinology. 2001 Jun;178(1-2):65-72. doi: 10.1016/s0303-7207(01)00411-7.

Fraietta R, Zylberstejn DS, Esteves SC. Hypogonadotropic hypogonadism revisited. Clinics (Sao Paulo). 2013;(68):81-8. doi: 10.6061/clinics/2013(sup01)09.